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Biblioteca (s) : |
INIA La Estanzuela. |
Fecha : |
12/02/2021 |
Actualizado : |
12/02/2021 |
Tipo de producción científica : |
Poster |
Autor : |
REGUEIRO, M; BALDI, F.; LÓPEZ MAZZ, C.; BANCHERO, G. |
Afiliación : |
M. REGUEIRO, Faculty of Agronomy, UDELAR, Garzón 780, 12900, Uruguay.; FERNANDO BALDI, UNESP, SP, 14884-900, Brazil,; CARLOS LÓPEZ MAZZ, Facultad de Agronomía, UDELAR.; GEORGGET ELIZABETH BANCHERO HUNZIKER, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Prepartum grazing with oat pasture and its effect on mother-lamb behaviour at parturition [Poster]. |
Complemento del título : |
Seccio 51- Poster 26 |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
In: Annual Meeting of the European Federation of Animal Science (EAAP), 69., 2018, Dubrovnik, Croatia. Book of abstracts... Wageningen, NL: WAP, 2018. |
Páginas : |
P. 521 |
Idioma : |
Inglés |
Contenido : |
Nutrition during gestation has an important role at time of parturition for both mother and offspring. The effect of different nutritional management during the last month of pregnancy on body condition (BCS), body weight (BW) and mother-lamb behaviour at parturition was evaluated. One hundred and forty multiparous Corriedale ewes gestating a single lamb were offered native pasture (7% crude protein (CP)) during the first four months of gestation and were assigned to two differential nutritional management during the last month of pregnancy: (1) ewes grazing oat pasture (14% CP) (GO, n=71); (2) ewes fed native pasture (GNP, n=69), regarding BW and BCS at the beginning
of the treatment (49.1±0.7 vs 49.7±0.7 kg and 3.74±0.05 vs 3.77±0.05 GO and GNP respectively). At parturition BW and BCS were registered, as well as duration of labour, delivery assistance and Maternal Behaviour Score (MBS,range 1-5). In lambs, birth weight, Apgar test for newborns (score 0-10), and time elapsed to stand and suck was recorded. Data were analysed using PROC MIXED and GENMOD of SAS, (mean ± SEM; P?0.05). At parturition GO ewes had higher BW (56.7±0.8 vs 52.7±0.7 kg P?0.0001) and BCS (3.7±0.04 vs 3.4±0.06 P=0.0007) than GNP. Duration of labour was longer (32.5±4.7 vs 24.03 min, p?0.001) and percentage of delivery assistance was significant
greater (22.5 vs 4.3%) in GO group. Lambs from GO mothers were heavier (4.77±0.07 vs 4.28±0.09 kg, P=0.0037),and took longer to stand (36.4±4.2 vs 28.5±3.9 min, p?0.001) and suck (56.4±5.2 vs 51.5±4.9 min, P=0.0011) than GNP lambs. There was no effect on MBS or Apgar test. The increment in BW and BCS of GO animals resulted in heavier lambs, that led to longer time of labor and less vigor compared with lambs of GNP ewes. Higher percentage of birth assistance in GO ewes suggests that the benefit of a greater weight at birth can be exploited only if control of parturition is performed. However, grazing oat pasture should be an alternative to be used with twin bearing ewes
since lambs are usually lighter and have less vigor than single ones. MenosNutrition during gestation has an important role at time of parturition for both mother and offspring. The effect of different nutritional management during the last month of pregnancy on body condition (BCS), body weight (BW) and mother-lamb behaviour at parturition was evaluated. One hundred and forty multiparous Corriedale ewes gestating a single lamb were offered native pasture (7% crude protein (CP)) during the first four months of gestation and were assigned to two differential nutritional management during the last month of pregnancy: (1) ewes grazing oat pasture (14% CP) (GO, n=71); (2) ewes fed native pasture (GNP, n=69), regarding BW and BCS at the beginning
of the treatment (49.1±0.7 vs 49.7±0.7 kg and 3.74±0.05 vs 3.77±0.05 GO and GNP respectively). At parturition BW and BCS were registered, as well as duration of labour, delivery assistance and Maternal Behaviour Score (MBS,range 1-5). In lambs, birth weight, Apgar test for newborns (score 0-10), and time elapsed to stand and suck was recorded. Data were analysed using PROC MIXED and GENMOD of SAS, (mean ± SEM; P?0.05). At parturition GO ewes had higher BW (56.7±0.8 vs 52.7±0.7 kg P?0.0001) and BCS (3.7±0.04 vs 3.4±0.06 P=0.0007) than GNP. Duration of labour was longer (32.5±4.7 vs 24.03 min, p?0.001) and percentage of delivery assistance was significant
greater (22.5 vs 4.3%) in GO group. Lambs from GO mothers were heavier (4.77±0.07 vs 4.28±0.09 kg, P=0.0037),and took longer to stand (36.4±4.2 vs 28.5±3.9 min,... Presentar Todo |
Palabras claves : |
REPRODUCCIÓN OVINA. |
Thesagro : |
OVINOS; REPRODUCCION ANIMAL. |
Asunto categoría : |
L53 Fisiología Animal - Reproducción |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/15006/1/EAAP-BOA-2018.Banchero.pdf
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Marc : |
LEADER 02779nam a2200193 a 4500 001 1061723 005 2021-02-12 008 2018 bl uuuu u00u1 u #d 100 1 $aREGUEIRO, M 245 $aPrepartum grazing with oat pasture and its effect on mother-lamb behaviour at parturition [Poster].$h[electronic resource] 260 $aIn: Annual Meeting of the European Federation of Animal Science (EAAP), 69., 2018, Dubrovnik, Croatia. Book of abstracts... Wageningen, NL: WAP$c2018 300 $aP. 521 520 $aNutrition during gestation has an important role at time of parturition for both mother and offspring. The effect of different nutritional management during the last month of pregnancy on body condition (BCS), body weight (BW) and mother-lamb behaviour at parturition was evaluated. One hundred and forty multiparous Corriedale ewes gestating a single lamb were offered native pasture (7% crude protein (CP)) during the first four months of gestation and were assigned to two differential nutritional management during the last month of pregnancy: (1) ewes grazing oat pasture (14% CP) (GO, n=71); (2) ewes fed native pasture (GNP, n=69), regarding BW and BCS at the beginning of the treatment (49.1±0.7 vs 49.7±0.7 kg and 3.74±0.05 vs 3.77±0.05 GO and GNP respectively). At parturition BW and BCS were registered, as well as duration of labour, delivery assistance and Maternal Behaviour Score (MBS,range 1-5). In lambs, birth weight, Apgar test for newborns (score 0-10), and time elapsed to stand and suck was recorded. Data were analysed using PROC MIXED and GENMOD of SAS, (mean ± SEM; P?0.05). At parturition GO ewes had higher BW (56.7±0.8 vs 52.7±0.7 kg P?0.0001) and BCS (3.7±0.04 vs 3.4±0.06 P=0.0007) than GNP. Duration of labour was longer (32.5±4.7 vs 24.03 min, p?0.001) and percentage of delivery assistance was significant greater (22.5 vs 4.3%) in GO group. Lambs from GO mothers were heavier (4.77±0.07 vs 4.28±0.09 kg, P=0.0037),and took longer to stand (36.4±4.2 vs 28.5±3.9 min, p?0.001) and suck (56.4±5.2 vs 51.5±4.9 min, P=0.0011) than GNP lambs. There was no effect on MBS or Apgar test. The increment in BW and BCS of GO animals resulted in heavier lambs, that led to longer time of labor and less vigor compared with lambs of GNP ewes. Higher percentage of birth assistance in GO ewes suggests that the benefit of a greater weight at birth can be exploited only if control of parturition is performed. However, grazing oat pasture should be an alternative to be used with twin bearing ewes since lambs are usually lighter and have less vigor than single ones. 650 $aOVINOS 650 $aREPRODUCCION ANIMAL 653 $aREPRODUCCIÓN OVINA 700 1 $aBALDI, F. 700 1 $aLÓPEZ MAZZ, C. 700 1 $aBANCHERO, G.
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INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Treinta y Tres. |
Fecha actual : |
21/02/2014 |
Actualizado : |
13/09/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - A |
Autor : |
NOYES, N.R.; WEINROTH, M.E.; PARKER, J.K.; DEAN, C.J.; LAKIN, S.M.; RAYMOND, R.A.; ROVIRA, P.J.; DOSTER, E.; ABDO, Z.; MARTIN, J.N.; JONES, K.L.; RUIZ, J.; BOUCHER, C.A.; BELK, K.E.; MORLEY, P.S. |
Afiliación : |
NOELLE R. NOYES; MAGGIE E. WEINROTH; JENNIFER K. PARKER; CHRIS J. DEAN; STEVEN M. LAKIN; ROBERT A. RAYMOND; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; ENRIQUE DOSTER; ZAID ABDO; JENNIFER N. MARTIN; KENNETH L. JONES; JAIME RUIZ; CHRISTINA A. BOUCHER; KEITH E. BELK; PAUL S. MORLEY. |
Título : |
Enrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing. |
Fecha de publicación : |
2017 |
Fuente / Imprenta : |
Microbiome, 2017, 5, p. 142 |
Páginas : |
13 p. |
DOI : |
10.1186/s40168-017-0361-8 |
Idioma : |
Inglés |
Notas : |
Article History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017 |
Contenido : |
Background: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins.
Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of
microbiome-resistome dynamics. MenosBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias.
Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional
metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistan... Presentar Todo |
Palabras claves : |
ANTIMICROBIAL RESISTANCE; METAGENÓMICA; MICROBIAL ECOLOGY; MOLECULAR ENRICHMENT; RARE MICROBIOME; RESISTOME. |
Thesagro : |
ANALISIS BIOLOGICO; ECOLOGIA MICROBIANA; RESISTENCIA A AGENTES DANINOS. |
Asunto categoría : |
U30 Métodos de investigación |
Marc : |
LEADER 03225naa a2200433 a 4500 001 1032862 005 2018-09-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1186/s40168-017-0361-8$2DOI 100 1 $aNOYES, N.R. 245 $aEnrichment allows identification of diverse, rate elements in metagenomic resistome-virulome sequencing.$h[electronic resource] 260 $c2017 300 $a13 p. 500 $aArticle History: Received: 29 May 2017, Accepted: 5 October 2017, Published: 17 October 2017 520 $aBackground: Shotgun metagenomic sequencing is increasingly utilized as a tool to evaluate ecological-level dynamics of antimicrobial resistance and virulence, in conjunction with microbiome analysis. Interest in use of this method for environmental surveillance of antimicrobial resistance and pathogenic microorganisms is also increasing. In published metagenomic datasets, the total of all resistance- and virulence-related sequences accounts for < 1% of all sequenced DNA, leading to imitations in detection of low-abundance resistome-virulome elements. This study describes the extent and composition of the low-abundance portion of the resistome-virulome, using a bait-capture and enrichment system that incorporates unique molecular indices to count DNA molecules and correct for enrichment bias. Results: The use of the bait-capture and enrichment system significantly increased on-target sequencing of the resistome-virulome, enabling detection of an additional 1441 gene accessions and revealing a low-abundance portion of the resistome-virulome that was more diverse and compositionally different than that detected by more traditional metagenomic assays. The low-abundance portion of the resistome-virulome also contained resistance genes with public health importance, such as extended-spectrum betalactamases, that were not detected using traditional shotgun metagenomic sequencing. In addition, the use of the bait-capture and enrichment system enabled identification of rare resistance gene haplotypes that were used to discriminate between sample origins. Conclusions: These results demonstrate that the rare resistome-virulome contains valuable and unique information that can be utilized for both surveillance and population genetic investigations of resistance. Access to the rare resistomevirulome using the bait-capture and enrichment system validated in this study can greatly advance our understanding of microbiome-resistome dynamics. 650 $aANALISIS BIOLOGICO 650 $aECOLOGIA MICROBIANA 650 $aRESISTENCIA A AGENTES DANINOS 653 $aANTIMICROBIAL RESISTANCE 653 $aMETAGENÓMICA 653 $aMICROBIAL ECOLOGY 653 $aMOLECULAR ENRICHMENT 653 $aRARE MICROBIOME 653 $aRESISTOME 700 1 $aWEINROTH, M.E. 700 1 $aPARKER, J.K. 700 1 $aDEAN, C.J. 700 1 $aLAKIN, S.M. 700 1 $aRAYMOND, R.A. 700 1 $aROVIRA, P.J. 700 1 $aDOSTER, E. 700 1 $aABDO, Z. 700 1 $aMARTIN, J.N. 700 1 $aJONES, K.L. 700 1 $aRUIZ, J. 700 1 $aBOUCHER, C.A. 700 1 $aBELK, K.E. 700 1 $aMORLEY, P.S. 773 $tMicrobiome, 2017, 5, p. 142
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